CRISPR-Cas: A Laboratory Manual by Jennifer Doudna
- CRISPR-Cas: A Laboratory Manual
- Jennifer Doudna
- Page: 450
- Format: pdf, ePub, mobi, fb2
- ISBN: 9781621821311
- Publisher: Cold Spring Harbor Laboratory Press
Download CRISPR-Cas: A Laboratory Manual
Free download ebooks for j2me CRISPR-Cas: A Laboratory Manual iBook FB2 CHM (English Edition)
CRISPR-Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for designing guide RNAs that precisely target the gene of interest, delivering CRISPR-Cas reagents into cells (e.g., using AAV vectors), and selecting cells that harbor the desired changes. Strategies for scaling and multiplexing these procedures are also described, as are methods for optimizing CRISPR-Cas systems in common species and cell lines, from yeast to human.
CRISPR-Cas and restriction–modification systems are compatible
Here, we show that R-M and CRISPR-Cas systems are compatible and J. & Russell, D. W. Molecular Cloning: A Laboratory Manual 3rd edn.
Cold Spring Harbor Lab Press Developmental Biology
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego.
Cold Spring Harbor Lab Press Genetics
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego.
Patent EP2764103B1 - Crispr-cas systems and methods for altering
An engineered, non-naturally occurring Type II CRISPR-Cas vector and Maniatis, MOLECULAR CLONING: A LABORATORY MANUAL,
The CRISPR web page - CSIC
Lluis Montoliu's lab at CNB-CSIC, Madrid, Spain. Multiplex genome engineering using CRISPR/Cas systems. Cong L, Ran FA, Cox D, Lin S,
2 - Cold Spring Harbor Lab Press
Budding Yeast: A Laboratory Manual Edited By Brenda Andrews, University of this Product · CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna,
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